capillary western blot analysis proteinsimple wes Search Results


capillary based protein detection system  (Protein Simple Inc)
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Protein Simple Inc capillary based protein detection system
Capillary Based Protein Detection System, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/capillary+western+blot+analysis+proteinsimple+wes/pm34323299-104-24-29?v=Protein+Simple+Inc
Average 97 stars, based on 1 article reviews
capillary based protein detection system - by Bioz Stars, 2026-07
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automatic western blot device  (Protein Simple Inc)
99
Protein Simple Inc automatic western blot device
FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an <t>automatic</t> capillary <t>Western</t> <t>blot</t> on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.
Automatic Western Blot Device, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/capillary+western+blot+analysis+proteinsimple+wes/pm38989059-256-7-14?v=Protein+Simple+Inc
Average 99 stars, based on 1 article reviews
automatic western blot device - by Bioz Stars, 2026-07
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wes automated capillary based immunoassay system  (Protein Simple Inc)
96
Protein Simple Inc wes automated capillary based immunoassay system
FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an <t>automatic</t> capillary <t>Western</t> <t>blot</t> on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.
Wes Automated Capillary Based Immunoassay System, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/capillary+western+blot+analysis+proteinsimple+wes/pm41671391-258-6-11?v=Protein+Simple+Inc
Average 96 stars, based on 1 article reviews
wes automated capillary based immunoassay system - by Bioz Stars, 2026-07
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capillary based sally sue instrument  (Protein Simple Inc)
94
Protein Simple Inc capillary based sally sue instrument
FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an <t>automatic</t> capillary <t>Western</t> <t>blot</t> on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.
Capillary Based Sally Sue Instrument, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
capillary based sally sue instrument - by Bioz Stars, 2026-07
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immunoassay system  (Protein Simple Inc)
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Protein Simple Inc immunoassay system
FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an <t>automatic</t> capillary <t>Western</t> <t>blot</t> on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.
Immunoassay System, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunoassay system - by Bioz Stars, 2026-07
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ice3 capillary ief system  (Protein Simple Inc)
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Protein Simple Inc ice3 capillary ief system
FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an <t>automatic</t> capillary <t>Western</t> <t>blot</t> on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.
Ice3 Capillary Ief System, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ice3 capillary ief system - by Bioz Stars, 2026-07
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immunoassay platform  (Protein Simple Inc)
97
Protein Simple Inc immunoassay platform
FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an <t>automatic</t> capillary <t>Western</t> <t>blot</t> on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.
Immunoassay Platform, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/capillary+western+blot+analysis+proteinsimple+wes/pmc11111483-205-8-10?v=Protein+Simple+Inc
Average 97 stars, based on 1 article reviews
immunoassay platform - by Bioz Stars, 2026-07
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immunoassay  (Protein Simple Inc)
94
Protein Simple Inc immunoassay
FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an <t>automatic</t> capillary <t>Western</t> <t>blot</t> on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.
Immunoassay, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cief immunoassay platform  (Protein Simple Inc)
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Protein Simple Inc cief immunoassay platform
FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an <t>automatic</t> capillary <t>Western</t> <t>blot</t> on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.
Cief Immunoassay Platform, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cief immunoassay platform - by Bioz Stars, 2026-07
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eclipse software sony  (Sony)
90
Sony eclipse software sony
FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an <t>automatic</t> capillary <t>Western</t> <t>blot</t> on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.
Eclipse Software Sony, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunoassay wes system  (Protein Simple Inc)
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Protein Simple Inc immunoassay wes system
FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an <t>automatic</t> capillary <t>Western</t> <t>blot</t> on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.
Immunoassay Wes System, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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maurice capillary electrophoresis instrument  (Protein Simple Inc)
96
Protein Simple Inc maurice capillary electrophoresis instrument
FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an <t>automatic</t> capillary <t>Western</t> <t>blot</t> on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.
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FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an automatic capillary Western blot on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.

Journal: Frontiers in cell and developmental biology

Article Title: Patient-derived and gene-edited pluripotent stem cells lacking NPHP1 recapitulate juvenile nephronophthisis in abnormalities of primary cilia and renal cyst formation.

doi: 10.3389/fcell.2024.1370723

Figure Lengend Snippet: FIGURE 1 Generation of NPHP1-overexpressing hiPSCs derived from patient-derived hiPSCs with whole NPHP1 deletion. (A) Schematic construct of pPB- CAG-NPHP1-PGK-HygR in DNA transposon. pPB: piggybac transposon plasmid, CAG: Cytomegalovirus (CMV) early enhancer element, the promoter, the first exon and the first intron of chicken beta-Actin gene, and the splice acceptor of the rabbit beta-Globin gene, PGK: phosphoglycerate kinase, HygR: hygromycin resistant gene. (B,C) Phase contrast images of patient-derived hiPSCs and their transfected cells treated with hygromycin at 50, 100, and 200 μg/mL for 5 days. Scale bars = 500 µm. (D) Relative mRNA expression of NPHP1 in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines. HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (E) Representative images of an automatic capillary Western blot on nephrocystin-1 protein in healthy donor hiPSCs (1383D6 line), patient-derived hiPSC lines, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. (F) Relative protein expression of nephrocystin-1 calculated from the data of automatic capillary Western blot. Each value was normalized with GAPDH value from the same sample. Data are shown as the mean ± SE (n = 3). (G,H) Immunocytochemistry of NPHP1 in patient-derived hiPSC line, HPS0447 and HPS0450, transfected hiPSC lines, HPS0447 NPHP1-OE and HPS0450 NPHP1-OE. Nuclei are stained with DAPI. Scale bars = 100 µm.

Article Snippet: Western blot was performed with a capillary automatic Western blot device (Simple Western, Wes; ProteinSimple) following the manufacturer’s instructions.

Techniques: Derivative Assay, Construct, Plasmid Preparation, Transfection, Expressing, Western Blot, Immunocytochemistry, Staining